Heat Inactivation of Coccidioides posadasii and Coccidioides immitis for Use in Lower Biosafety Containment
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چکیده
منابع مشابه
Taxonomic and diagnostic markers for identification of Coccidioides immitis and Coccidioides posadasii.
The ribosomal Internal Transcribed Spacer (ITS) regions of the two recognized species of Coccidioides were studied using a reference set of strains that had been previously identified with species defining microsatellite polymorphisms. Unambiguous identification of the two species proved to be possible by amplifying and sequencing the ITS region. PCR-reactions are sensitive to amplification con...
متن کاملGenetic analysis of the endemic fungal pathogens Coccidioides posadasii and Coccidioides immitis in Mexico.
Coccidioidomycosis (CM) is a mycotic disease that affects mammals, including humans. Official data relative to CM in Mexico has not been collected since 1995, thus its prevalence remains unknown. The objectives of this study were to identify the predominant Coccidioides species in Mexico, infer their current geographical distribution and explore the correlation between species and clinical pres...
متن کاملUse of the Coccidioides posadasii Deltachs5 strain for quality control in the ACCUPROBE culture identification test for Coccidioides immitis.
Coccidioides posadasii Deltachs5 is a strain that is excluded from the select agent list. Sixteen assays using test reagents from three different ACCUPROBE Coccidioides immitis culture identification test lots had an average of 132,998 relative light units (RLU), which is well beyond the 50,000-RLU positive cutoff value for the test. Coccidioides posadasii Deltachs5 is a satisfactory quality co...
متن کاملPolysaccharide of Coccidioides immitis.
Pappagianis, D. (University of California, Berkeley), E. W. Putman, and G. S. Kobayashi. Polysaccharide of Coccidioides immitis. J. Bacteriol. 82:714-723. 1961.-Soluble polysaccharide from mycelia or culture filtrates of Coccidioides immitis was found to consist mainly of mannose, but also included small quantities of galactose and another reducing sugar. Isolation of the polysaccharide by etha...
متن کاملMolecular and phenotypic description of Coccidioides posadasii sp. nov., previously recognized as the non-California population of Coccidioides immitis.
Coccidioides posadasii sp. nov., formerly known as non-California (non-CA) Coccidioides immitis, is described. Phylogenetic analyses using single nucleotide polymorphisms, genes, and microsatellites show that C. posadasii represents a divergent, genetically recombining monophyletic clade. Coccidioides posadasii can be distinguished from C. immitis by numerous DNA polymorphisms, and we show how ...
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ژورنال
عنوان ژورنال: Applied Biosafety
سال: 2019
ISSN: 1535-6760,2470-1246
DOI: 10.1177/1535676019856525